Microbiological surveillance of hospital environment in tertiary care hospital in north India

Saboor Naik, Mohammad Abass Dar, Sufhia Khan, Kanika Razdhan


Background: Hospital acquired infection (nosocomial infection) are commonly occurring in developing as well as developed countries almost 50% of patients in developed countries and 75% in developing countries get infected inside the hospital to which they are not exposed earlier. The problem occurring in treatment to these patients is the resistance to certain antibiotics among these bacteria.

Methods: A swab soaked in nutrient broth was used to collect samples from the floor, walls, equipments, instruments, operation tables, etc. All the samples were labelled properly and immediately transported to the microbiology laboratory in Government medical college Jammu for processing.

Results: Out of 29 air samples positive is 9 and the percent is 31.0 swabs are 92. Out of 92 samples positive is 28 and the percent is 30.43. Out of 29 samples 3 samples are taken from orthopaedic OT there is no growth. 3 samples are taken from gynaecology OT no growth. 4 samples are taken from urology theatre 2 are Staphylococcus aureus and the percent is 50. 2 samples are taken from eye OT there is no growth.

Conclusions: These findings demonstrate that the microbiological quality of air and surfaces in operation theatre may be considered a mirror image of the hygienic conditions of the operating theatre.


Nosocomial, Proteus, Bacterial, Air, Surveillance, Infection

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Slaughter S, Hayden MK, Nathan C, Hu TC, Rice T, Van Voorhis J, et al. A comparison of the effect of universal use of gloves and gowns with that of glove use alone on acquisition of vancomycin-resistant enterococci in a medical intensive care unit. Ann Intern Med. 1996;125(6):448-56.

Bonten MJ, Hayden MK, Nathan C, van Voorhis J, Matushek M, Slaughter S, et al. Epidemiology of colonisation of patients and environment with vancomycin-resistant enterococci. Lancet. 1996;348(9042):1615-9.

Vincent JL, Bihari DJ, Suter PM, Bruining HA, White J, Nicolas-Chanoin MH, et al. The prevalence of nosocomial infection in intensive care units in Europe. Results of the European Prevalence of Infection in Intensive Care (EPIC) Study. EPIC International Advisory Committee. JAMA. 1995;274(8):639-44.

Vincent JL, Rello J, Marshall J, Silva E, Anzueto A, Martin CD, et al. International study of the prevalence and outcomes of infection in intensive care units. JAMA. 2009;302(21):2323-9.

Haley RW, Culver DH, White JW, Morgan WM, Emori TG, Munn VP, et al. The efficacy of infection surveillance and control programs in preventing nosocomial infections in US hospitals. Am J Epidemiol. 1985;121(2):182-205.

Ananthanarayan S, Panikaer A. Text book of Microbiology. 7th ed. New Delhi: Jaypee Publishers; 634.

Shukla A, Srivastava S, Srivastava A, Srivastava T. Surveillance of Microbiological Environment of Operation Theaters. Cureus. 2021;13(12):e20525.

Singh K, Kishore K. Surveillance of Microbiological Environment of Operation Theaters. Am J Epidemiol. 2021;13(12):e20525.

Galvin S, Dolan A, Cahill O, Daniels S, Humphreys H. Microbial monitoring of the hospital environment: why and how? J Hosp Infect. 2012;82(3):143-51.

Sikora A, Zahra F. Nosocomial Infections. In: StatPearls. Treasure Island (FL): StatPearls Publishing; 2022.

Slaughter S, Hayden MK, Nathan C, Hu TC, Rice T, Van Voorhis J, et al. A comparison of the effect of universal use of gloves and gowns with that of glove use alone on acquisition of vancomycin-resistant enterococci in a medical intensive care unit. Ann Intern Med. 1996; 125(6):448-56.

Revelas A. Healthcare - associated infections: A public health problem. Niger Med J. 2012;53(2):59-64.

Fridkin SK, Welbel SF, Weinstein RA. Magnitude and prevention of nosocomial infections in the intensive care unit. Infect Dis Clin North Am. 1997;11(2):479-96.

Pittet D, Allegranzi B, Sax H, Bertinato L, Concia E, Cookson B, et al. Considerations for a WHO European strategy on health-care-associated infection, surveillance, and control. Lancet Infect Dis. 2005; 5(4):242-50.

Leape LL, Brennan TA, Laird N, Lawthers AG, Localio AR, Barnes BA, et al. The nature of adverse events in hospitalized patients. Results of the Harvard Medical Practice Study II. N Engl J Med. 1991;324(6):377-84.

ohn AH, Garrett DO, Sinkowitz-Cochran RL, Grohskopf LA, Levine GL, Stover BH, et al. Pediatric Prevention Network. Prevalence of nosocomial infections in neonatal intensive care unit patients: Results from the first national point-prevalence survey. J Pediatr. 2001;139(6):821-7.

Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. PT. 2015;40(4):277-83.

Klevens RM, Edwards JR, Richards CL Jr, Horan TC, Gaynes RP, Pollock DA, et al. Estimating health care-associated infections and deaths in U.S. hospitals, 2002. Public Health Rep. 2007;122(2):160-6.

Andrew P. The antibiotic resistance crisis. J Pediatr. 2015;40(4):277-83.

Liziolia A, Privitera G, Alliata E, Antonietta Banfi EM, Boselli L, Panceri ML, et al. Prevalence of nosocomial infections in Italy. N Engl J Med. 2003;3:141.

Lyytikäinen O, Kanerva M, Agthe N, Möttönen T, Ruutu P. Finnish Prevalence Survey Study Group. Healthcare-associated infections in Finnish acute care hospitals: a national prevalence survey, 2005. J Hosp Infect. 2008;69(3):288-94.

CDC surveillance update. Atlanta: Centers for Disease Control and Prevention. Available at: ttps://www.cdc. gov. Accessed on 20 November 2021.

Suzuki A, Namba Y, Matsuura M, Horisawa A. Bacterial contamination of floors and other surfaces in operating rooms; a five years suvey. J Hyg Camb. 1984;93;559-66.

Hughes SPF, Anderson FM. Infection in the Operating Room. J. Bone Joint Surg. 1999;81:754-5.

Javed I, Hafeez, R, Zubair M, Anwar MS, Husnain S. Microbiological surveillance of operation theatres and ICUs of atertiary hospital, Lahore. Biomedica. 2008;24;99-102.

Khan MA, Ansari MN, Bano S. Post operative wound infection. India J Surg. 1985;48:383-6.

Mawalla B, Mshana SE, Chalya PL, Mirzalioglu C, Mahlu W. Prebuictors of Surgical site infection among patients under going major surgery at Bugando Medical Centre in North Western Tanzaniya. BMC Sug. 2011;11:21.