Test the effectiveness of antibacterial effect of the skin of the pomegranate fruit (Punica granatum) extract against the growth of Escherichia coli in vitro

Authors

  • Niken . Department of Public Health, Health Science Institute Syedza Saintika, Indonesia
  • Eliza Arman Department of Public Health, Health Science Institute Syedza Saintika, Indonesia
  • Rahmi Novita Yusuf Department of Public Health, Health Science Institute Syedza Saintika, Indonesia
  • Honesty Diana Morika Department of Public Health, Health Science Institute Syedza Saintika, Indonesia

DOI:

https://doi.org/10.18203/2394-6040.ijcmph20201966

Keywords:

Antibacterial, Escherichia coli, Pomegranate

Abstract

Background: Pomegranate (Punica granatum) is one of the traditional medicines that contains antibacterial compounds that are effective against bacterial growth. Its chemical content includes saponins, tannins and flavonoids. Escherichia coli is a gram-negative bacterium which is a normal flora germ found in the human large intestine. These bacteria are pathogenic when they are outside the intestine and produce enterotoxins in epithelial cells which cause diarrhea. The purpose of this study was to determine the antibacterial effect of pomegranate skin extract on the growth of Escherichia coli.

Methods: This study used an experimental design study with a complete randomized design study divided into 5 groups namely groups 1 (15 ug/ml), 2 (20 ug/ml), 3 (25 ug/ml), positive control (ciprofloxatin), negative control. Making pomegranate peel extract was done by maceration method then rotary, after that the effectiveness of pomegranate extract extracted by the diffusion method was tested using Anova one-way test.

Results: The results showed that the extract of pomegranate peel showed that it was able to inhibit the growth of Escherichia coli with a ratio of constants (15 ug/ml, 20 ug/ml, 25 ug/ml with inhibition diameters of 6.7 mm, 6.7 mm, 6, 7 mm, while for positive control with ciprofloxatin showed bacterial resistance to antibiotics.

Conclusions: Statistically, pomegranate skin extract has an antibacterial power which is meaningful with p 0.005. Pomegranate rind extract has antibacterial activity against Escherichia coli medium because inhibition zone is 5-10 mm.

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Published

2020-04-24

How to Cite

., N., Arman, E., Yusuf, R. N., & Morika, H. D. (2020). Test the effectiveness of antibacterial effect of the skin of the pomegranate fruit (Punica granatum) extract against the growth of Escherichia coli in vitro. International Journal Of Community Medicine And Public Health, 7(5), 1680–1683. https://doi.org/10.18203/2394-6040.ijcmph20201966

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Original Research Articles